eIF2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84879

Novus Biologicals, part of Bio-Techne
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NBP1-84879
NBP1-84879-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF2A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSTEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2A Antibody

Western Blot: eIF2A Antibody [NBP1-84879]

Western Blot: eIF2A Antibody [NBP1-84879]

Western Blot: eIF2A Antibody [NBP1-84879] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: eIF2A Antibody [NBP1-84879]

Immunocytochemistry/ Immunofluorescence: eIF2A Antibody [NBP1-84879]

Immunocytochemistry/Immunofluorescence: eIF2A Antibody [NBP1-84879] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: eIF2A Antibody [NBP1-84879]

Immunohistochemistry-Paraffin: eIF2A Antibody [NBP1-84879]

Immunohistochemistry-Paraffin: eIF2A Antibody [NBP1-84879] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
View 1 more images

Applications for eIF2A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

1:100-1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF2A

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Alternate Names

EIF-2, EIF-2A, EIF2AEIF2, eIF-2alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, subunit 1 (alpha, 35kD )

Gene Symbol

EIF2A

Additional eIF2A Products

Product Documents for eIF2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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