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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 400 and 450 of human eukaryotic translation initiation factor 2B, subunit 5 epsilon using the numbering given in entry NP_003898.2 (GeneID 8893).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2B epsilon Antibody - BSA Free

Western Blot: eIF2B epsilon Antibody [NBP1-30448]

Western Blot: eIF2B epsilon Antibody [NBP1-30448]

Western Blot: eIF2B epsilon Antibody [NBP1-30448] - Western Blot and Immunoprecipitation: Detection of Human eIF2B5 Whole cell lysate from HeLa and 293 cells. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30449.
Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448]

Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448]

Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for eIF2B epsilon Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eIF2B epsilon

The eIF2B epsilon gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other E

Alternate Names

CACH, CLE, EIF-2B, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2BE, EIF2Bepsilon, eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD), eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, LVWM, translation initiation factor eIF-2B subunit epsilon

Gene Symbol

EIF2B5

Additional eIF2B epsilon Products

Product Documents for eIF2B epsilon Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2B epsilon Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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