Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all eIF2B epsilon Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 400 and 450 of human eukaryotic translation initiation factor 2B, subunit 5 epsilon using the numbering given in entry NP_003898.2 (GeneID 8893).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF2B epsilon Antibody

Western Blot: eIF2B epsilon Antibody [NBP1-30448]

Western Blot: eIF2B epsilon Antibody [NBP1-30448]

Western Blot: eIF2B epsilon Antibody [NBP1-30448] - Western Blot and Immunoprecipitation: Detection of Human eIF2B5 Whole cell lysate from HeLa and 293 cells. eIF2B5 was also immunoprecipitated by rabbit anti-eIF2B5 antibody NBP1-30449.
Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448]

Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448]

Immunohistochemistry: eIF2B epsilon Antibody [NBP1-30448] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF2B5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for eIF2B epsilon Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

5-15 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eIF2B epsilon

The eIF2B epsilon gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other E

Alternate Names

CACH, CLE, EIF-2B, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2BE, EIF2Bepsilon, eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD), eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, LVWM, translation initiation factor eIF-2B subunit epsilon

Gene Symbol

EIF2B5

Additional eIF2B epsilon Products

Product Documents for eIF2B epsilon Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF2B epsilon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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