eIF2B3 Antibody (OTI1A4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71397

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all eIF2B3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EIF2B3(NP_065098) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2B3 Antibody (OTI1A4) - Azide and BSA Free

Western Blot: eIF2B3 Antibody (OTI1A4)Azide and BSA Free [NBP2-71397]

Western Blot: eIF2B3 Antibody (OTI1A4)Azide and BSA Free [NBP2-71397]

Western Blot: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2B3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eIF2B3.
Immunocytochemistry/ Immunofluorescence: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397]

Immunocytochemistry/ Immunofluorescence: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397]

Immunocytochemistry/Immunofluorescence: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2B3.
Immunohistochemistry: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397]

Immunohistochemistry: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397]

Immunohistochemistry: eIF2B3 Antibody (OTI1A4) - Azide and BSA Free [NBP2-71397] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2B3 mouse monoclonal antibody.
View 5 more images

Applications for eIF2B3 Antibody (OTI1A4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF2B3

The protein encoded by the eIF2B3 gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Alternate Names

EIF-2B, eIF-2B GDP-GTP exchange factor subunit gamma, EIF2Bgamma, eukaryotic translation initiation factor 2B, subunit 3 (gamma, 58kD), eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa, translation initiation factor eIF-2B subunit gamma

Gene Symbol

EIF2B3

Additional eIF2B3 Products

Product Documents for eIF2B3 Antibody (OTI1A4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for eIF2B3 Antibody (OTI1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.