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EIF2G Antibody (2C9)

Novus Biologicals, part of Bio-Techne | Catalog # H00001968-M01

Novus Biologicals, part of Bio-Techne
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H00001968-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2C9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EIF2S3 (NP_001406, 383 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAVKADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD

Specificity

EIF2S3 - eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa (2C9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EIF2G Antibody (2C9)

Western Blot: EIF2G Antibody (2C9) [H00001968-M01]

Western Blot: EIF2G Antibody (2C9) [H00001968-M01]

Western Blot: EIF2G Antibody (2C9) [H00001968-M01] - Analysis of EIF2S3 expression in transfected 293T cell line by EIF2S3 monoclonal antibody (M01), clone 2C9.Lane 1: EIF2S3 transfected lysate(51.1 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: EIF2G Antibody (2C9) [H00001968-M01]

Immunocytochemistry/ Immunofluorescence: EIF2G Antibody (2C9) [H00001968-M01]

Immunocytochemistry/Immunofluorescence: EIF2G Antibody (2C9) [H00001968-M01] - Analysis of monoclonal antibody to EIF2S3 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: EIF2G Antibody (2C9) [H00001968-M01] - Detection limit for recombinant GST tagged EIF2S3 is 1 ng/ml as a capture antibody.

Applications for EIF2G Antibody (2C9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EIF2G

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiatortRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex.Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound toeIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round ofinitiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B

Alternate Names

EIF2, eIF-2gA, EIF2gamma, eIF-2-gamma X, EIF2Geukaryotic translation initiation factor 2, subunit 3 (gamma, 52kD), eIF-2gX, eukaryotic translation initiation factor 2 subunit 3, Eukaryotic translation initiation factor 2 subunit gamma X, eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa, eukaryotic translation initiation factor 2G

Entrez Gene IDs

1968 (Human)

Gene Symbol

EIF2S3

Additional EIF2G Products

Product Documents for EIF2G Antibody (2C9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EIF2G Antibody (2C9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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