eIF3e Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30440

Novus Biologicals, part of Bio-Techne
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NBP3-30440
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF3e Antibodies »

Product Specifications

Immunogen

between 1 and 50

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF3e Antibody

eIF3e Antibody

Western Blot:[NBP3-30440]-

Western Blot:[NBP3-30440]-Detection of human and mouse eIF3E by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-eIF3E antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 3 µg/mg lysate. eIF3E was also immunoprecipitated by rabbit anti-eIF3E antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes and 10 seconds.

Applications for eIF3e Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: eIF3e

Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stressresponsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Alternate Names

eIF-3 p48, eIF3e, eIF3-p48, EIF3S6EIF3-P48, Eukaryotic translation initiation factor 3 subunit 6, eukaryotic translation initiation factor 3 subunit E, eukaryotic translation initiation factor 3, subunit 6 (48kD), eukaryotic translation initiation factor 3, subunit 6 48kDa, eukaryotic translation initiation factor 3, subunit E, INT6eIF3-p46, mammary tumor-associated protein INT6, murine mammary tumor integration site 6 (oncogene homolog), Viral integration site protein INT-6 homolog

Gene Symbol

EIF3E

Additional eIF3e Products

Product Documents for eIF3e Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF3e Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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