EIF3F Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50037
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Chimpanzee (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 307 and 357 of human Eukaryotic Translation Initiation Factor 3, Subunit F using the numbering given in entry NP_003745.1 (GeneID 8665).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for EIF3F Antibody
![Western Blot: EIF3F Antibody [NBP1-50037]](https://resources.bio-techne.com/images/products/EIF3F-Antibody-Western-Blot-NBP1-50037-img0003.jpg "Western Blot: EIF3F Antibody [NBP1-50037]")
Western Blot: EIF3F Antibody [NBP1-50037]
Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope. For blotting immunoprecipitated eIF3F, the ReliaBLOT (R) Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).![Immunohistochemistry-Paraffin: EIF3F Antibody [NBP1-50037]](https://resources.bio-techne.com/images/products/EIF3F-Antibody-Immunohistochemistry-NBP1-50037-img0006.jpg "Immunohistochemistry-Paraffin: EIF3F Antibody [NBP1-50037]")
Immunohistochemistry-Paraffin: EIF3F Antibody [NBP1-50037]
Immunohistochemistry-Paraffin: EIF3F Antibody [NBP1-50037] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- eIF3F used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).Applications for EIF3F Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: EIF3F
Alternate Names
eIF3 p47, eIF-3-epsilon, eIF3-epsilon, eIF3f, eIF3-p47, EIF3S5eukaryotic translation initiation factor 3 subunit F, Eukaryotic translation initiation factor 3 subunit 5, eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD), eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa, eukaryotic translation initiation factor 3, subunit F
Gene Symbol
EIF3F
Additional EIF3F Products
Product Documents for EIF3F Antibody
Product Specific Notices for EIF3F Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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