EIF3F Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77997
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Chimpanzee (100%), Drosophila (90%), Monkey (100%), Mouse (100%), Pufferfish (90%). Backed by our 100% Guarantee.
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified EIF3F Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 100-125 of human EIF3F protein. (Uniprot: O00303)
Reactivity Notes
A BLAST analysis was used to suggest cross reactivity with EIF3F protein from human, mouse, rat, chimpanzee, chicken, and bovine based on 100% homology with the immunizing sequence. Partial reactivity is expected against EIF3F from Drosophila melanogaster and Tetraodon nigroviridis (pufferfish) based on 90% homology of the immunizing sequence with EIF3F from these sources. Reactivity against homologues from other sources is not known.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity-purified antibody is directed against human EIF3F protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for EIF3F Antibody
Western Blot: EIF3F Antibody [NBP1-77997]
Western Blot: EIF3F Antibody [NBP1-77997] - shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.Applications for EIF3F Antibody
Application
Recommended Usage
ELISA
1:10000-1:40000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:200-1:2000
Application Notes
This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size by western blotting in the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: EIF3F
Alternate Names
eIF3 p47, eIF-3-epsilon, eIF3-epsilon, eIF3f, eIF3-p47, EIF3S5eukaryotic translation initiation factor 3 subunit F, Eukaryotic translation initiation factor 3 subunit 5, eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD), eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa, eukaryotic translation initiation factor 3, subunit F
Gene Symbol
EIF3F
UniProt
Additional EIF3F Products
Product Documents for EIF3F Antibody
Product Specific Notices for EIF3F Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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