EIF3S3 Antibody (GT35512)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13550

Novus Biologicals, part of Bio-Techne
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NBP3-13550
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (98%), Chicken (95%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # GT35512

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EIF3S3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EIF3S3. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (86%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for EIF3S3 Antibody (GT35512)

Western Blot: EIF3S3 Antibody (GT35512) [NBP3-13550]

Western Blot: EIF3S3 Antibody (GT35512) [NBP3-13550]

Western Blot: EIF3S3 Antibody (GT35512) [NBP3-13550] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EIF3S3 antibody [GT35512] (NBP3-13550) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: EIF3S3 Antibody (GT35512) [NBP3-13550]

Immunocytochemistry/ Immunofluorescence: EIF3S3 Antibody (GT35512) [NBP3-13550]

Immunocytochemistry/Immunofluorescence: EIF3S3 Antibody (GT35512) [NBP3-13550] - EIF3S3 antibody [GT35512] detects EIF3S3 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3S3 protein stained by EIF3S3 antibody [GT35512] (NBP3-13550) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: EIF3S3 Antibody (GT35512) [NBP3-13550]

Immunohistochemistry-Paraffin: EIF3S3 Antibody (GT35512) [NBP3-13550]

Immunohistochemistry-Paraffin: EIF3S3 Antibody (GT35512) [NBP3-13550] - Mouse testis. EIF3S3 antibody [35512] (NBP3-13550) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for EIF3S3 Antibody (GT35512)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF3S3

Eukaryotic initiation factor 3 subunit H (eIF3H) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3H is a non-conserved subunit and part of the functional core of eIF3. It may function to stabilize the eIF3 complex and stimulate protein synthesis. Many cancer cell lines have been shown to express high levels of eIF3H, and it may have an oncogenic role in colorectal cancer susceptibility.

Alternate Names

eIF-3-gamma, eIF3-gamma, eIF3h, eIF3-p40, EIF3S3eIF3 p40 subunit, Eukaryotic translation initiation factor 3 subunit 3, eukaryotic translation initiation factor 3 subunit H, eukaryotic translation initiation factor 3, subunit 2 (beta, 36kD), eukaryotic translation initiation factor 3, subunit 3 (gamma, 40kD), eukaryotic translation initiation factor 3, subunit 3 gamma, 40kDa, eukaryotic translation initiation factor 3, subunit H, MGC102958

Gene Symbol

EIF3H

Additional EIF3S3 Products

Product Documents for EIF3S3 Antibody (GT35512)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EIF3S3 Antibody (GT35512)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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