EIF3S3 Antibody (PSH04-49)

Western Blot: EIF3S3 Antibody (PSH04-49) [NBP3-32298]

Western Blot: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Western blot analysis of EIF3S3 on different lysates with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/2,000 dilution.

Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: SK-Br-3 cell lysate
Lane 8: MDA-MB-231 cell lysate
Lane 9: MDA-MB-468 cell lysate
Lane 10: MCF7 cell lysate
Lane 11: RAW264.7 cell lysate
Lane 12: NIH/3T3 cell lysate
Lane 13: PC-12 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 40 kDa
Observed band size: 40 kDa

Exposure time: 3 minutes; ECL;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32298) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: EIF3S3 Antibody (PSH04-49) [NBP3-32298]

Immunohistochemistry: EIF3S3 Antibody (PSH04-49) [NBP3-32298] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3S3 antibody (NBP3-32298) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32298) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.