eIF4E [p Ser209] Antibody (1F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26426

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1F3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4E Antibodies »

Product Summary for eIF4E [p Ser209] Antibody (1F3)

Immunogen

A synthesized peptide derived from Human EIF4E [p Ser209] [UniProt P06730]

Modification

p Ser 209

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4E [p Ser209] Antibody (1F3)

eIF4E [p Ser209] Antibody (1F3)

Immunohistochemistry: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] -

Immunohistochemistry: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Image of eIF4E [p Ser209] Antibody (1F3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
eIF4E [p Ser209] Antibody (1F3)

Western Blot: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] -

Western Blot: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Positive Western Blot detected in 293 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or not).
All lanes: eIF4E [p Ser209] Antibody at 1.2ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 25 KDa
Observed band size: 25 KDa

Applications for eIF4E [p Ser209] Antibody (1F3)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E [p Ser209] Antibody (1F3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF4E [p Ser209] Antibody (1F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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