eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08884

Novus Biologicals, part of Bio-Techne
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NBP3-08884
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # PCRP-EIF4E-1D3

Format

Azide and BSA Free

Concentration

1 mg/ml

View all eIF4E Antibodies »

Product Summary for eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

Immunogen

Recombinant full-length human eIF4E protein (Uniprot: P06730)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07141).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884]

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884]

Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).
Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884]

Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Flow cytometric analysis of PFA-fixed U87 cells. eIF4E antibody (PCRP-EIF4E-1D3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).
Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884]

Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884]

Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).
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Applications for eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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