eIF4E Antibody (PCRP-EIF4E-1D3) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08884PE

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP3-07141

Alexa Fluor 350

NBP3-08884AF350

Alexa Fluor 405

NBP3-08884AF405

Alexa Fluor 488

NBP3-08884AF488

Alexa Fluor 532

NBP3-08884AF532

Alexa Fluor 594

NBP3-08884AF594

Alexa Fluor 647

NBP3-08884AF647

Alexa Fluor 700

NBP3-08884AF700

Alexa Fluor 750

NBP3-08884AF750

Allophycocyanin

NBP3-08884APC

Biotin

NBP3-08884B

CoraFluor 1

NBP3-08884CL1

DyLight 350

NBP3-08884UV

DyLight 405

NBP3-08884V

DyLight 488

NBP3-08884G

DyLight 550

NBP3-08884R

DyLight 594

NBP3-08884DL594

DyLight 650

NBP3-08884C

DyLight 680

NBP3-08884FR

DyLight 755

NBP3-08884IR

FITC

NBP3-08884F

HRP

NBP3-08884H

Janelia Fluor 525

NBP3-08884JF525

Janelia Fluor 549

NBP3-08884JF549

Janelia Fluor 646

NBP3-08884JF646

Janelia Fluor 669

NBP3-08884JF669

mFluor Violet 450 SE

NBP3-08884MFV450

mFluor Violet 500 SE

NBP3-08884MFV500

mFluor Violet 610 SE

NBP3-08884MFV610

PerCP

NBP3-08884PCP

Forumulation

Catalog #

Azide and BSA Free

NBP3-08884

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG_2A Clone # PCRP-EIF4E-1D3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4E Antibodies »

Product Specifications

Immunogen

Recombinant full-length human eIF4E protein (Uniprot: P06730)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for eIF4E Antibody (PCRP-EIF4E-1D3) [PE]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Protein Array

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E Antibody (PCRP-EIF4E-1D3) [PE]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for eIF4E Antibody (PCRP-EIF4E-1D3) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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Flow Cytometry