eIF4E Antibody (RM452)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23456

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-23456
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM452

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eIF4E Antibodies »

Product Summary for eIF4E Antibody (RM452)

Immunogen

A phospho-peptide corresponding to human eIF4E [Ser209] (Uniprot#: P06730 )

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4E Antibody (RM452)

eIF4E Antibody (RM452)

Western Blot: eIF4E Antibody (RM452) [NBP3-23456] -

Western Blot: eIF4E Antibody (RM452) [NBP3-23456] - Western Blot of the lysates of MCF-7 cells nontreated (-) or treated (+) with fetal bovine serum, using Clone RM452 at a 1:1000 dilution.

Applications for eIF4E Antibody (RM452)

Application
Recommended Usage

Western Blot

1:1000 -1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF4E

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Long Name

Eukaryotic Translation Initiation Factor 4E

Alternate Names

EIF4EL1, EIF4F

Gene Symbol

EIF4E

Additional eIF4E Products

Product Documents for eIF4E Antibody (RM452)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for eIF4E Antibody (RM452)

[Public Notes]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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