eIF4G1 Antibody (3A10)
Novus Biologicals, part of Bio-Techne | Catalog # H00001981-M01
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3A10
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EIF4G1 (NP_886553, 1500 a.a. ~ 1599 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
EIF4G1 - eukaryotic translation initiation factor 4 gamma, 1 (3A10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for eIF4G1 Antibody (3A10)
![Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01]](https://resources.bio-techne.com/images/products/eIF4G1-Antibody-3A10-Western-Blot-H00001981-M01-img0005.jpg "Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01]")
Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01]
Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01] - EIF4G1 monoclonal antibody (M01), clone 3A10. Analysis of EIF4G1 expression in NIH/3T3.![Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01]](https://resources.bio-techne.com/images/products/eIF4G1-Antibody-3A10-Western-Blot-H00001981-M01-img0004.jpg "Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01]")
Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01]
Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01] - EIF4G1 monoclonal antibody (M01), clone 3A10. Analysis of EIF4G1 expression in IMR-32.![ELISA: eIF4G1 Antibody (3A10) [H00001981-M01]](https://resources.bio-techne.com/images/products/eIF4G1-Antibody-3A10-ELISA-H00001981-M01-img0006.jpg "ELISA: eIF4G1 Antibody (3A10) [H00001981-M01]")
ELISA: eIF4G1 Antibody (3A10) [H00001981-M01]
ELISA: eIF4G1 Antibody (3A10) [H00001981-M01] - Detection limit for recombinant GST tagged EIF4G1 is 0.03 ng/ml as a capture antibody.Applications for eIF4G1 Antibody (3A10)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: eIF4G1
Long Name
Eukaryotic Translation Initiation Factor 4 gamma 1
Alternate Names
DKFZp686A1451, EIF4F, eIF-4G 1, eIF-4G1, EIF4-gamma, eIF-4-gamma 1, EIF4GI, EIF4Gp220, eucaryotic translation initiation factor 4G, eukaryotic translation initiation factor 4 gamma 1, eukaryotic translation initiation factor 4 gamma, 1, P220
Entrez Gene IDs
1981 (Human)
Gene Symbol
EIF4G1
UniProt
Additional eIF4G1 Products
Product Documents for eIF4G1 Antibody (3A10)
Product Specific Notices for eIF4G1 Antibody (3A10)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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