eIF4G1 [p Ser1231] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04965

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-04965
NBP1-04965-0.025ml
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all eIF4G1 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human eIF4G1 [p Ser1231] around the phosphorylation site of serine 1231 (P-V-Sp-P-L).

Modification

p Ser1231

Specificity

Detects endogenous levels of eIF4G1 only when phosphorylated at serine 1231.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF4G1 [p Ser1231] Antibody

Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] -

Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NBP1-04965 or the same antibody preincubated with blocking peptide(right).
Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965]

Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965]

Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.
Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965]

Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965]

Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.

Applications for eIF4G1 [p Ser1231] Antibody

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NBP1-04965 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4G1

eIF4G1 (eukaryotic translation Initiation Factor 4 Gamma 1) is a component of the protein complex eIF-4 which is involved in the recognition of the mRNA cap ATP-dependent unwinding of the 5'-terminal secondary structure and recruitment of mRNA to the ribosome. eIF4G plays a critical role in protein expression and is at the center of a complex regulatory network. Together with the cap-binding protein eIF4E, it recruits the small ribosomal subunit to the 5'-end of mRNA and promotes the assembly of a functional translation initiation complex which scans along the mRNA to the translation start codon. Human eIF4G contains three consecutive HEAT domains, as well as long unstructured regions involved in multiple protein-protein interactions. The interactions of eIF4G1 with other factors are largely unknown.

Long Name

Eukaryotic Translation Initiation Factor 4 gamma 1

Alternate Names

DKFZp686A1451, EIF4F, eIF-4G 1, eIF-4G1, EIF4-gamma, eIF-4-gamma 1, EIF4GI, EIF4Gp220, eucaryotic translation initiation factor 4G, eukaryotic translation initiation factor 4 gamma 1, eukaryotic translation initiation factor 4 gamma, 1, P220

Entrez Gene IDs

1981 (Human)

Gene Symbol

EIF4G1

UniProt

Q04637

Additional eIF4G1 Products

Product Documents for eIF4G1 [p Ser1231] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for eIF4G1 [p Ser1231] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.