eIF4GII Antibody (1H5)

Novus Biologicals, part of Bio-Techne | Catalog # H00008672-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF4GII Antibodies »

Product Specifications

Immunogen

EIF4G3 (AAH30578, 1 a.a. ~ 515 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNSQPQTRSPFAAGPRPPHHQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIMVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIISGGGSRNPTPPIGRPTSTPTPPQQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC

Specificity

EIF4G3 - eukaryotic translation initiation factor 4 gamma, 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for eIF4GII Antibody (1H5)

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03]

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03]

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of EIF4G3 expression in MCF-7 (Cat # L046V1).
Immunohistochemistry-Paraffin: eIF4GII Antibody (1H5) [H00008672-M03]

Immunohistochemistry-Paraffin: eIF4GII Antibody (1H5) [H00008672-M03]

Immunohistochemistry-Paraffin: eIF4GII Antibody (1H5) [H00008672-M03] - Analysis of monoclonal antibody to EIF4G3 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml
Western Blot: eIF4GII Antibody (1H5) [H00008672-M03]

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03]

Western Blot: eIF4GII Antibody (1H5) [H00008672-M03] - EIF4G3 over-expressed 293 cell line, cotransfected with EIF4G3 Validated Chimera RNAi or non-transfected control. Blot probed with H00008672-M03. GAPDH (36.1 kDa) used as loading control.
View 3 more images

Applications for eIF4GII Antibody (1H5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF4GII

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1

Alternate Names

eIF-4G 3, eIF-4-gamma 3, eIF-4-gamma II, eIF4GIIeIF4G 3, eukaryotic translation initiation factor 4 gamma 3, eukaryotic translation initiation factor 4 gamma, 3

Entrez Gene IDs

8672 (Human)

Gene Symbol

EIF4G3

OMIM

603929 (Human)

UniProt

AAH30578 (Human)

Additional eIF4GII Products

Product Documents for eIF4GII Antibody (1H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4GII Antibody (1H5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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