eIF4GII Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84867

Novus Biologicals, part of Bio-Techne
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NBP1-84867
NBP1-84867-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF4GII Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EFDSRRTLTSRGSMGREKNDKPLPSATARPNTFMRGGSSKDLLDNQSQEEQRREMLETVKQLTGGVDVERNSTEAERNKTRESAKPEISAMSA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for eIF4GII Antibody

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP1-84867]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP1-84867]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP1-84867] - Staining in human testis and skeletal muscle tissues using anti-EIF4G3 antibody. Corresponding EIF4G3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP1-84867]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP1-84867]

Immunohistochemistry-Paraffin: eIF4GII Antibody [NBP1-84867] - Staining of human cerebral cortex, lymph node, skeletal muscle and testis using Anti-EIF4G3 antibody NBP1-84867 (A) shows similar protein distribution across tissues to independent antibody NBP1-84865 (B).
Immunocytochemistry/ Immunofluorescence: eIF4GII Antibody [NBP1-84867]

Immunocytochemistry/ Immunofluorescence: eIF4GII Antibody [NBP1-84867]

Immunocytochemistry/Immunofluorescence: eIF4GII Antibody [NBP1-84867] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
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Applications for eIF4GII Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval method is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eIF4GII

Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1

Alternate Names

eIF-4G 3, eIF-4-gamma 3, eIF-4-gamma II, eIF4GIIeIF4G 3, eukaryotic translation initiation factor 4 gamma 3, eukaryotic translation initiation factor 4 gamma, 3

Gene Symbol

EIF4G3

Additional eIF4GII Products

Product Documents for eIF4GII Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eIF4GII Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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