ELA3A Antibody (3G4)

Novus Biologicals, part of Bio-Techne | Catalog # H00010136-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3G4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ELA3A Antibodies »

Product Specifications

Immunogen

ELA3A (AAH07028, 16 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SGYGPPSSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSGFGCNFIWKPTVFTRVSAFIDWIEETIASH

Reactivity Notes

Other species not tested.

Specificity

ELA3A - elastase 3A, pancreatic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for ELA3A Antibody (3G4)

Western Blot: ELA3A Antibody (3G4) [H00010136-M02]

Western Blot: ELA3A Antibody (3G4) [H00010136-M02]

Western Blot: ELA3A Antibody (3G4) [H00010136-M02] - ELA3A monoclonal antibody (M02), clone 3G4. Analysis of ELA3A expression in human pancreas.
Immunohistochemistry-Paraffin: ELA3A Antibody (3G4) [H00010136-M02]

Immunohistochemistry-Paraffin: ELA3A Antibody (3G4) [H00010136-M02]

Immunohistochemistry-Paraffin: ELA3A Antibody (3G4) [H00010136-M02] - Analysis of monoclonal antibody to ELA3A on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Sandwich ELISA: ELA3A Antibody (3G4) [H00010136-M02] - Detection limit for recombinant GST tagged ELA3A is approximately 1ng/ml as a capture antibody.

Applications for ELA3A Antibody (3G4)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ELA3A

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1.

Alternate Names

chymotrypsin-like elastase family member 3A, chymotrypsin-like elastase family, member 3A, EC 3.4.21, EC 3.4.21.70, ELA3A, ELA3elastase 1, elastase 3A, pancreatic, elastase 3A, pancreatic (protease E), Elastase IIIA, elastase-3A, Protease E

Entrez Gene IDs

10136 (Human)

Gene Symbol

CELA3A

UniProt

AAH07028 (Human)

Additional ELA3A Products

Product Documents for ELA3A Antibody (3G4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ELA3A Antibody (3G4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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