EMAP-II/AIMP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26124

Novus Biologicals, part of Bio-Techne
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NBP2-26124
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all EMAP-II Antibodies »

Product Specifications

Immunogen

Peptide with sequence NNDAVLKRLEQK-C corresponding to N-Terminus according to NP_001135888.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_004748.2; NP_001135888.1). Reported variants represent identical protein: NP_004748.2, NP_001135887.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EMAP-II/AIMP1 Antibody

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-26124]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-26124]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-26124] - NBP2-26124 (0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for EMAP-II/AIMP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 37 kDa band observed in lysates of HeLa and Jurkat, and in lysates of mouse and rat spleen (calculated MW of 37 kDa band according to NP_001135887.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EMAP-II

EMAP II (Endothelial-Monocyte-Activating Polypeptide II) is a proinflammatory cytokine and chemoattractant of macrophages and polymorphonuclear lymphocytes. EMAP II is synthesized as a 34 kDa precursor molecule which is cleaved to a biologically active 22 kDa mature polypeptide. This active protein is known to induce the procoagulant protein tissue factor on the surface of endothelial cells and modulate other properties of endothelial cells and monocytes in vitro, including the stimulation of TNF-alpha and myeloperoxidase. EMAP II activates neutrophils in vivo and induces an inflammatory reaction and tumor regression. EMAP II induces apoptosis in proliferating endothelial cells and inhibits neovascularization. This anti-angiogenic property is of interest to tumor biology. EMAP II is also a negative modulator of lung vascular growth.

Long Name

Endothelial-Monocyte Activating Polypeptide II

Alternate Names

AIMP1, EMAPII, SCYE1

Entrez Gene IDs

9255 (Human); 13722 (Mouse); 114632 (Rat)

Gene Symbol

AIMP1

Additional EMAP-II Products

Product Documents for EMAP-II/AIMP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EMAP-II/AIMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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