EMAP-II/AIMP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98343

Novus Biologicals, part of Bio-Techne
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NBP2-98343-100ul
NBP2-98343-50ul
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EMAP-II Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Mouse EMAP-II/AIMP1 (Uniprot#: Q62386; Ser145-Lys310)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EMAP-II/AIMP1 Antibody

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-98343]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-98343]

Western Blot: EMAP-II/AIMP1 Antibody [NBP2-98343] - Anti-EMAP-II/AIMP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Rat brain Tissue Lysate. Lane C: 293T Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 38 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: EMAP-II/AIMP1 Antibody [NBP2-98343]

Immunoprecipitation: EMAP-II/AIMP1 Antibody [NBP2-98343]

Immunoprecipitation: EMAP-II/AIMP1 Antibody [NBP2-98343] - Mouse EMAP-II/AIMP1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Mouse EMAP-II/AIMP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Mouse EMAP-II/AIMP1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa

Applications for EMAP-II/AIMP1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EMAP-II

EMAP II (Endothelial-Monocyte-Activating Polypeptide II) is a proinflammatory cytokine and chemoattractant of macrophages and polymorphonuclear lymphocytes. EMAP II is synthesized as a 34 kDa precursor molecule which is cleaved to a biologically active 22 kDa mature polypeptide. This active protein is known to induce the procoagulant protein tissue factor on the surface of endothelial cells and modulate other properties of endothelial cells and monocytes in vitro, including the stimulation of TNF-alpha and myeloperoxidase. EMAP II activates neutrophils in vivo and induces an inflammatory reaction and tumor regression. EMAP II induces apoptosis in proliferating endothelial cells and inhibits neovascularization. This anti-angiogenic property is of interest to tumor biology. EMAP II is also a negative modulator of lung vascular growth.

Long Name

Endothelial-Monocyte Activating Polypeptide II

Alternate Names

AIMP1, EMAPII, SCYE1

Gene Symbol

AIMP1

Additional EMAP-II Products

Product Documents for EMAP-II/AIMP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EMAP-II/AIMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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