EMMPRIN/CD147 Antibody (8D6)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33015
![Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) [NBP2-33015]](https://resources.bio-techne.com/images/products/EMMPRIN-CD147-Antibody-8D6-Immunohistochemistry-Paraffin-NBP2-33015-img0001.jpg "Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) [NBP2-33015]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 8D6
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human T cell line Molt 13
Localization
Cell surface
Specificity
This monoclonal antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34543)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for EMMPRIN/CD147 Antibody (8D6)
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) [NBP2-33015]
Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) [NBP2-33015] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD147 Monoclonal Antibody (8D6).Applications for EMMPRIN/CD147 Antibody (8D6)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Frozen
0.5-1.0ug/ml
Immunohistochemistry-Paraffin
0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migration through Matrigel: Order Ab without BSA/Azide.
Optimal dilution for a specific application should be determined.
Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migration through Matrigel: Order Ab without BSA/Azide.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: EMMPRIN/CD147
Long Name
Extracellular Matrix Metalloproteinase Inducer
Alternate Names
Basigin, BSG, CD147
Gene Symbol
BSG
UniProt
Additional EMMPRIN/CD147 Products
Product Documents for EMMPRIN/CD147 Antibody (8D6)
Product Specific Notices for EMMPRIN/CD147 Antibody (8D6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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