EN-RAGE/S100A12 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-90136

Novus Biologicals, part of Bio-Techne
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NB110-90136
NB110-90136SS
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Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all EN-RAGE/S100A12 Antibodies »

Product Specifications

Immunogen

Full length human S100A12 protein [Swiss-Prot# P80511] expressed in E. coli.

Reactivity Notes

Human. Not reactive with mouse or rat.

Localization

Nuclear and Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EN-RAGE/S100A12 Antibody

Western Blot: EN-RAGE/S100A12 Antibody [NB110-90136]

Western Blot: EN-RAGE/S100A12 Antibody [NB110-90136]

Western Blot: S100A12 Antibody [NB110-90136] - Detection of S100A12 in HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: EN-RAGE/S100A12 Antibody [NB110-90136]

Immunocytochemistry/ Immunofluorescence: EN-RAGE/S100A12 Antibody [NB110-90136]

Immunocytochemistry/Immunofluorescence: S100A12 Antibody [NB110-90136] - S100A12 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Applications for EN-RAGE/S100A12 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:1000
Application Notes
This S100A12 antibody is useful for Western blot, where a band is seen at ~14 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EN-RAGE/S100A12

S100A12 (Calgranulin C, MRP6, or EN-RAGE) is a calcium-binding pro-inflammatory protein that belongs to S100 family of proteins (family named so as its members are 100% solubile in ammonium sulfate at normal pH) and its expression is restricted to neutrophil granulocytes and monocytes. In normal individuals, it is expresses in tissue/organs where neutrophils and monocytes/macrophages are common, such as spleen and lung, wherein, it is located predominantly in granulocyte cytosol but translocates to membrane as well as cytoskeletal components following interactions with calcium. S100A12 involves in recruitment of leukocytes, promotion of cytokine/chemokine production, and regulation of leukocyte adhesion as well as migration. It acts as an alarmin or DAMP (danger associated molecular pattern) molecule for stimulation of innate immune cells via binding to receptor for AGER that follows activation of MAPK and NFkB signaling leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1/VCAM1. Additionally, it been suggested to inhibit matrix metalloproteinases activity (of MMP2, MMP3 and MMP9 by chelating Zn2+ from their active sites) and also to acts as a monocyte/mast cell chemoattractant, and involve in triggering mast cell degranulation as well as activation that generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation.

Long Name

Extracellular Newly Identified RAGE-binding Protein

Alternate Names

CAAF1, CAAFI, CAGC, CAGCS100, Calgranulin C, ENRAGE, MRP6, S100A12

Entrez Gene IDs

6283 (Human)

Gene Symbol

S100A12

UniProt

P80511

Additional EN-RAGE/S100A12 Products

Product Documents for EN-RAGE/S100A12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EN-RAGE/S100A12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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