ENC1 Antibody (3B1)

Novus Biologicals, part of Bio-Techne | Catalog # H00008507-M02

Novus Biologicals, part of Bio-Techne
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H00008507-M02
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ENC1 Antibodies »

Product Specifications

Immunogen

ENC1 (NP_003624, 17 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SINIYLFHKSSYADSVLTHLNLLRQQRLFTDVLLHAGNRTFPCHRAVLAACSRYFEAMFSGGLKESQDSEVNFDNSIHPEVL

Specificity

ENC1 - ectodermal-neural cortex (with BTB-like domain)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for ENC1 Antibody (3B1)

Western Blot: ENC1 Antibody (3B1) [H00008507-M02]

Western Blot: ENC1 Antibody (3B1) [H00008507-M02]

Western Blot: ENC1 Antibody (3B1) [H00008507-M02] - ENC1 monoclonal antibody (M02), clone 3B1 Analysis of ENC1 expression in IMR-32.
Sandwich ELISA: ENC1 Antibody (3B1) [H00008507-M02] - Detection limit for recombinant GST tagged ENC1 is approximately 0.3ng/ml as a capture antibody.

Applications for ENC1 Antibody (3B1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: ENC1

DNA damage and/or hyperproliferative signals activate wildtype p53 tumor suppressor protein (TP53; MIM 191170), inducing cell cycle arrest or apoptosis. Mutations that inactivate p53 occur in 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] used serial analysis of gene expression (SAGE) to evaluate cellular mRNA levels in a colorectal cancer cell line transfected with p53. Of 7,202 transcripts identified, only 14 were expressed at levels more than 10-fold higher in p53-expressing cells than in control cells. Polyak et al. (1997) [PubMed 9305847] termed these genes 'p53-induced genes,' or PIGs, several of which were predicted to encode redox-controlling proteins. They noted that reactive oxygen species (ROS) are potent inducers of apoptosis. Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions. The authors stated that the PIG10 gene, also called ENC1, encodes an actin-binding protein.[supplied by OMIM]

Alternate Names

CCL28, ectodermal-neural cortex 1 (with BTB-like domain), ENC-1BTB domain-like (brain), FLJ39259, PIG10KLHL35, tumor protein p53 inducible protein 10

Entrez Gene IDs

8507 (Human)

Gene Symbol

ENC1

OMIM

605173 (Human)

UniProt

NP_003624 (Human)

Additional ENC1 Products

Product Documents for ENC1 Antibody (3B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ENC1 Antibody (3B1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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