Endoglin/CD105 Antibody (MJ7/18) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81085

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP2-81085AF350

Alexa Fluor 405

NBP2-81085AF405

Alexa Fluor 488

NBP2-81085AF488

Alexa Fluor 532

NBP2-81085AF532

Alexa Fluor 594

NBP2-81085AF594

Alexa Fluor 647

NBP2-81085AF647

Alexa Fluor 700

NBP2-81085AF700

Alexa Fluor 750

NBP2-81085AF750

Allophycocyanin

NBP2-81085APC

Biotin

NBP2-81085B

CoraFluor 1

NBP2-81085CL1

DyLight 350

NBP2-81085UV

DyLight 405

NBP2-81085V

DyLight 488

NBP2-81085G

DyLight 550

NBP2-81085R

DyLight 594

NBP2-81085DL594

DyLight 650

NBP2-81085C

DyLight 680

NBP2-81085FR

DyLight 755

NBP2-81085IR

FITC

NBP2-81085F

HRP

NBP2-81085H

Janelia Fluor 525

NBP2-81085JF525

Janelia Fluor 549

NBP2-81085JF549

Janelia Fluor 646

NBP2-81085JF646

Janelia Fluor 669

NBP2-81085JF669

mFluor Violet 450 SE

NBP2-81085MFV450

mFluor Violet 500 SE

NBP2-81085MFV500

mFluor Violet 610 SE

NBP2-81085MFV610

PE

NBP2-81085PE

PerCP

NBP2-81085PCP

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MJ7/18

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Endoglin/CD105 Antibodies »

Product Specifications

Immunogen

MJ7/18 was prepared by immunizing rats with inflamed mouse skin tissue. The antibody was selected immunohistologically for reactivity with endothelial cells.

Specificity

MJ7/18 binds specifically to mouse Endoglin/CD105. Endoglin/CD105 is a homodimer expressed by vascular endothelial cells. Endoglin/CD105 functions in adhesion and embryonic angiogenesis. Endoglin/CD105 is a major homodimeric glycoprotein of vascular endothelium involved in cell ahesion through integrin/RGD binding and the regulation of angiogenesis. Also acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Endoglin/CD105 is also required for GDF2/BMP9 signaling through SMAD1 in endothelial cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Endoglin/CD105 Antibody (MJ7/18) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric - Azide and BSA Free [NBP2-81085]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) - Chimeric [NBP2-81085] - Immunofluorescence staining of fixed mouse splenocytes with Endoglin/CD105 Antibody (MJ7/18) [NBP2-81085]. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of MJ7/18 [NBP2-81085] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining in a small subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81085, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for Endoglin/CD105 Antibody (MJ7/18) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. MJ7/18 can be used for FC, WB, IP and IHC (Baluk P, et al. 2003. Am. J. Pathol. 163:1801).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Endoglin/CD105

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and up-regulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF- szlig1, TGF- szlig3 and modulates TGF- szlig signaling by interacting with TGF- szlig receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2 and -7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging, prognosis, as well as has therapeutic potential for some solid tumors and other angiogenic diseases.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MJ7/18) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for Endoglin/CD105 Antibody (MJ7/18) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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