Endothelial Lipase Antibody (1E11)

Novus Biologicals, part of Bio-Techne | Catalog # H00009388-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Endothelial Lipase Antibodies »

Product Specifications

Immunogen

LIPG (AAH60825.1, 21 a.a. ~ 500 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGQELWFRKCRDGWRMKNETSPTVELP

Specificity

LIPG - lipase, endothelial

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Endothelial Lipase Antibody (1E11)

Western Blot: Endothelial Lipase Antibody (1E11) [H00009388-M02]

Western Blot: Endothelial Lipase Antibody (1E11) [H00009388-M02]

Western Blot: Endothelial Lipase Antibody (1E11) [H00009388-M02] - Analysis of LIPG expression in transfected 293T cell line by LIPG monoclonal antibody (M02), clone 1E11.Lane 1: LIPG transfected lysate(56.8 KDa).Lane 2: Non-transfected lysate.
ELISA: Endothelial Lipase Antibody (1E11) [H00009388-M02]

ELISA: Endothelial Lipase Antibody (1E11) [H00009388-M02]

ELISA: Endothelial Lipase Antibody (1E11) [H00009388-M02] - Detection limit for recombinant GST tagged LIPG is approximately 1ng/ml as a capture antibody.

Applications for Endothelial Lipase Antibody (1E11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Endothelial Lipase

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family.

Alternate Names

EC 3.1.1, EC 3.1.1.3, EDLendothelial lipase, ELlipoprotein lipase H, Endothelial cell-derived lipase, lipase, endothelial, PRO719

Entrez Gene IDs

9388 (Human)

Gene Symbol

LIPG

OMIM

603684 (Human)

UniProt

AAH60825 (Human)

Additional Endothelial Lipase Products

Product Documents for Endothelial Lipase Antibody (1E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Endothelial Lipase Antibody (1E11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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