Endothelin-1 Antibody (3D6)
Novus Biologicals, part of Bio-Techne | Catalog # H00001906-M01
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3D6
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EDN1 (AAH09720, 113 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGNPSRERYVTHNRAHW
Specificity
EDN1 - endothelin 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Endothelin-1 Antibody (3D6)
![Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01]](https://resources.bio-techne.com/images/products/Endothelin-1-Antibody-3D6-Western-Blot-H00001906-M01-img0005.jpg "Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01]")
Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01]
Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 expression in transfected 293T cell line by EDN1 monoclonal antibody (M01), clone 3D6.Lane 1: EDN1 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.![Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01]](https://resources.bio-techne.com/images/products/Endothelin-1-Antibody-3D6-Immunoprecipitation-H00001906-M01-img0003.jpg "Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01]")
Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01]
Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 transfected lysate using anti-EDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Endothelin-1 Antibody (3D6) [H00001906-M01] - Detection limit for recombinant GST tagged EDN1 is approximately 0.3ng/ml as a capture antibody.
Applications for Endothelin-1 Antibody (3D6)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Endothelin-1
Alternate Names
ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME
Entrez Gene IDs
1906 (Human)
Gene Symbol
EDN1
OMIM
131240 (Human)
UniProt
Additional Endothelin-1 Products
Product Documents for Endothelin-1 Antibody (3D6)
Product Specific Notices for Endothelin-1 Antibody (3D6)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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