Endothelin-1 Antibody (TR.ET.48.5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB300-526

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine, Rabbit, Sheep

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Radioimmunoassay, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TR.ET.48.5

Format

BSA Free

Concentration

2.5 mg/ml

View all Endothelin-1 Antibodies »

Product Specifications

Immunogen

Pure endothelin-1 conjugated to KLH.

Epitope

Studies suggest that this antibody binds to an epitope in the region of ET-1 represented by amino acids 8-16.

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 11673223). Rabbit reactivity reported in scientific literature (PMID: 12181591). Porcine reactivity reported in scientific literature (PMID: 21587116). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Endothelin-1 Antibody (TR.ET.48.5) - BSA Free

Western Blot: Endothelin-1 Antibody (TR.ET.48.5)BSA Free [NB300-526]

Western Blot: Endothelin-1 Antibody (TR.ET.48.5)BSA Free [NB300-526]

Western Blot: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of 25 ug of PC12 cell lysates.
Immunocytochemistry/ Immunofluorescence: Endothelin-1 Antibody (TR.ET.48.5) - BSA Free [NB300-526]

Immunocytochemistry/ Immunofluorescence: Endothelin-1 Antibody (TR.ET.48.5) - BSA Free [NB300-526]

Immunocytochemistry/Immunofluorescence: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Analysis of Endothelin 1 (green) showing positive staining in the secretion of Hela cells (right) compared with a negative control in the absence of primary antibody (left).
Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) - BSA Free [NB300-526]

Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) - BSA Free [NB300-526]

Immunohistochemistry: Endothelin-1 Antibody (TR.ET.48.5) [NB300-526] - Immunolocalization of ET-1 in human bowel.
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Applications for Endothelin-1 Antibody (TR.ET.48.5) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 ug

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:250

Immunohistochemistry-Frozen

1:250

Immunohistochemistry-Paraffin

1:250

Radioimmunoassay

1:25000

Western Blot

1:100 - 1:1000
Application Notes
IHC: Staining of ET-1 in human corpus cavernosum tissue with this antibody results in staining of endothelial cells. Radioimmune assays can be used to concentrate ET-1 in solution (e.g. serum/plasma, milk, urine).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB300-526 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

2.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Endothelin-1

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.

Alternate Names

ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME

Entrez Gene IDs

1906 (Human)

Gene Symbol

EDN1

UniProt

P05305 (Human)

Additional Endothelin-1 Products

Product Documents for Endothelin-1 Antibody (TR.ET.48.5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endothelin-1 Antibody (TR.ET.48.5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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