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engrailed homeobox 2 Antibody (1E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00002020-M03

Novus Biologicals, part of Bio-Techne
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H00002020-M03

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EN2 (NP_001418.2, 86 a.a. ~ 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTCCAGAGGGRGGGAGGEGGASGAEGGGGAGGSEQLLGSGSREPRQNPPCAPGAGGPLPAAGSDSPGDGEGGSKTLSLHGGAKKGGDPGGPLDGSLKARGLGGGDLSVSSDSDSSQAGANLGAQP

Specificity

EN2 - engrailed homolog 2 (1E1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for engrailed homeobox 2 Antibody (1E1)

Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody (1E1) [H00002020-M03]

Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody (1E1) [H00002020-M03]

Immunocytochemistry/Immunofluorescence: engrailed homeobox 2 Antibody (1E1) [H00002020-M03] - Immunofluorescence of monoclonal antibody to engrailed homeobox 2 on HeLa cell. [antibody concentration 40 ug/ml]
Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody (1E1) [H00002020-M03]

Immunocytochemistry/ Immunofluorescence: engrailed homeobox 2 Antibody (1E1) [H00002020-M03]

Immunocytochemistry/Immunofluorescence: engrailed homeobox 2 Antibody (1E1) [H00002020-M03] - Analysis of monoclonal antibody to EN2 on HeLa cell. Antibody concentration 40 ug/ml
Sandwich ELISA: engrailed homeobox 2 Antibody (1E1) [H00002020-M03] - Detection limit for recombinant GST tagged EN2 is 3 ng/ml as a capture antibody.

Applications for engrailed homeobox 2 Antibody (1E1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Engrailed-2

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq]

Alternate Names

EN2, Engrailed2

Entrez Gene IDs

2020 (Human)

Gene Symbol

EN2

Additional Engrailed-2 Products

Product Documents for engrailed homeobox 2 Antibody (1E1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for engrailed homeobox 2 Antibody (1E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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