ENO3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85821

Novus Biologicals, part of Bio-Techne
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NBP1-85821
NBP1-85821-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (96%), Rat (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ENO3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ENO3 Antibody

Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-85821]

Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-85821]

Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-85821] - Staining in human skeletal muscle and tonsil tissues using anti-ENO3 antibody. Corresponding ENO3 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: ENO3 Antibody [NBP1-85821]

Immunocytochemistry/ Immunofluorescence: ENO3 Antibody [NBP1-85821]

Immunocytochemistry/Immunofluorescence: ENO3 Antibody [NBP1-85821] - Staining of human cell line U-2 OS shows localization to plasma membrane and cytosol.
Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-85821]

Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-85821]

Immunohistochemistry-Paraffin: ENO3 Antibody [NBP1-85821] - Staining of human skeletal muscle shows high expression.
View 1 more images

Applications for ENO3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENO3

ENO3 encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

Alternate Names

2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, beta-enolase, EC 4.2.1, EC 4.2.1.11, Enolase 3, enolase 3 (beta, muscle), enolase 3, (beta, muscle), GSD13, MSE, Muscle-specific enolase, Skeletal muscle enolase

Gene Symbol

ENO3

Additional ENO3 Products

Product Documents for ENO3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ENO3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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