ENO3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00266

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ENO3 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ENO3 (Accession#: AAH17249.1; Met1-Lys434)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ENO3 Antibody

Western Blot: ENO3 Antibody [NBP3-00266]

Western Blot: ENO3 Antibody [NBP3-00266]

Western Blot: ENO3 Antibody [NBP3-00266] - Anti-ENO3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: MOLT4 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: ENO3 Antibody [NBP3-00266]

Immunoprecipitation: ENO3 Antibody [NBP3-00266]

Immunoprecipitation: ENO3 Antibody [NBP3-00266] - ENO3 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 2 ul anti-ENO3 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-ENO3 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa

Applications for ENO3 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ENO3

ENO3 encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.

Alternate Names

2-phospho-D-glycerate hydrolyase, 2-phospho-D-glycerate hydro-lyase, beta-enolase, EC 4.2.1, EC 4.2.1.11, Enolase 3, enolase 3 (beta, muscle), enolase 3, (beta, muscle), GSD13, MSE, Muscle-specific enolase, Skeletal muscle enolase

Gene Symbol

ENO3

Additional ENO3 Products

Product Documents for ENO3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ENO3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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