Enolase 1 Antibody (1G7) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59457
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1G7
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human Enolase 1 (1-434aa) purified from E. coli
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Enolase 1 Antibody (1G7) - BSA Free
Western Blot: Enolase 1 Antibody (1G7)BSA Free [NBP2-59457]
Western Blot: Enolase 1 Antibody (1G7) [NBP2-59457] - The recombinant Enolase 1 protein [NBP1-30262] (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human [NBP2-59457] (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ENO1 (Alpha-enolase) recombinant protein.Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody (1G7) - BSA Free [NBP2-59457]
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ENO1, 2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).Flow Cytometry: Enolase 1 Antibody (1G7) - BSA Free [NBP2-59457]
Flow Cytometry: Enolase 1 Antibody (1G7) [NBP2-59457] - Analysis of ENO1, 2 in LNCap cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).Applications for Enolase 1 Antibody (1G7) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1059
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4), 10% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enolase 1
Alternate Names
2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin
Gene Symbol
ENO1
Additional Enolase 1 Products
Product Documents for Enolase 1 Antibody (1G7) - BSA Free
Product Specific Notices for Enolase 1 Antibody (1G7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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