Enolase 1 Antibody (253)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25147
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Equine
Cited:
Human
Applications
Validated:
Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunoassay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 253
Concentration
1 mg/ml
Product Specifications
Immunogen
The N-terminal 12 amino acids of bovine Enolase 1, which was synthesized on a 8-amine lysine core. [UniProt# Q9XSJ4]
Localization
Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Enolase 1 Antibody (253)
Western Blot: Enolase 1 Antibody (253) [NBP2-25147]
Western Blot: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of different cell lysates using mouse mAb to alpha-enolase, NBP2-25147, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the alpha-enolase protein.Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147]
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147] - Analysis of HeLa cells stained with mouse mAb to alpha-enolase, NBP2-25147, dilution 1:500 in green and costained with chicken pAb to HSP60, dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. NBP2-25147 antibody reveals strong cytoplasmic staining.Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147]
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (253) [NBP2-25147] - Rat 3T3 cells stained with NBP2-25147 (green) and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (red) and DNA (blue). NBP2-25147 reveals strong cytoplasmic staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments.Applications for Enolase 1 Antibody (253)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:2000 - 1:5000
Immunohistochemistry
1:2000 - 1:5000
Simple Western
1:500
Western Blot
1:5000 - 1:10000
Application Notes
This Enolase 1 (253) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approx. 47 kDa. Use in Immmunoassay reported in scientific literature (PMID: 31035430).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50% PBS, 50% glycerol
Preservative
0.035% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enolase 1
Alternate Names
2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin
Entrez Gene IDs
2023 (Human)
Gene Symbol
ENO1
OMIM
172430 (Human)
UniProt
Additional Enolase 1 Products
Product Documents for Enolase 1 Antibody (253)
Product Specific Notices for Enolase 1 Antibody (253)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...