Enolase 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65252
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Cited:
Mouse, Bovine
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
5 mg/ml
Product Specifications
Immunogen
Native human Enolase 1
Reactivity Notes
Bovine reactivity reported in scientific literature (PMID: 21051714). Use in Mouse reported in scientific literature (PMID:28495754).
Localization
Cytoplasm. Cell membrane. Isoform MBP-1: Nucleus. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Specificity
This recognises human Non-neuronal enolase, and demostrates less than 0.1% cross-reactivity with non-specific enolase. Non-neuronal enolase is a glycolytic enzyme composed of homodimeric alpha enolase subunits and is expressed in most human tissues. The gene coding for the 48kDa form of non-neuronal enolase also codes for a shorter 37kDa isoform, myc-binding protein 1 (MBP-1) lacking the N-terminal 93 amino acids of non-neuronal enolase.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Enolase 1 Antibody
![Immunohistochemistry: Enolase 1 Antibody [NB100-65252]](https://resources.bio-techne.com/images/products/Enolase-1-Antibody-Immunohistochemistry-NB100-65252-img0004.jpg "Immunohistochemistry: Enolase 1 Antibody [NB100-65252]")
Immunohistochemistry: Enolase 1 Antibody [NB100-65252]
Immunohistochemistry: Enolase 1 Antibody [NB100-65252] - Staining of Jurkat cells with rabbit anti human Enolase 1 .![Simple Western: Enolase 1 Antibody [NB100-65252]](https://resources.bio-techne.com/images/products/Enolase-1-Antibody-Simple-Western-NB100-65252-img0003.jpg "Simple Western: Enolase 1 Antibody [NB100-65252]")
Simple Western: Enolase 1 Antibody [NB100-65252]
Simple Western: Enolase 1 Antibody [NB100-65252] - Simple Western lane view shows a specific band for Enolase 1 in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for Enolase 1 Antibody
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Frozen
1:25 - 1:100
Immunohistochemistry-Paraffin
1:25 - 1:100
Simple Western
1:5000
Western Blot
1:100 - 1:2000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.09% Sodium Azide
Concentration
5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Enolase 1
Alternate Names
2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin
Entrez Gene IDs
2023 (Human)
Gene Symbol
ENO1
UniProt
Additional Enolase 1 Products
Product Documents for Enolase 1 Antibody
Product Specific Notices for Enolase 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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