Enolase 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16337
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (96%), Chicken (92%), Guinea Pig (95%), Primate (100%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Enolase 1. The exact sequence is proprietary.
Reactivity Notes
Chimpanzee (100%), Xenopus laevis (92%).
Localization
Cytoplasm; Cell membrane; myofibril; sarcomere; M-band; Isoform MBP-1: Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Enolase 1 Antibody
Western Blot: Enolase 1 Antibody [NBP2-16337]
Western Blot: Enolase 1 Antibody [NBP2-16337] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Enolase 1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Enolase 1 Antibody [NBP2-16337]
Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody [NBP2-16337] - Immunofluorescence analysis of methanol-fixed HCT116, using antibody at 1:500 dilution.Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337]
Immunohistochemistry-Paraffin: Enolase 1 Antibody [NBP2-16337] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.Applications for Enolase 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:500-1:1000
Western Blot
1:5000-1:20000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Enolase 1
Alternate Names
2-phospho-D-glycerate hydro-lyase, Alpha enolase, C-myc promoter-binding protein, c-myc promoter-binding protein-1, EC 4.2.1, EC 4.2.1.11, ENO1L1, Enolase 1, enolase 1, (alpha), MBP-1, MBPB1, MPB-1, MPB1c-myc promoter-binding protein 1, MYC promoter-binding protein 1, NNE, Non-neural enolase, Phosphopyruvate hydratase, Plasminogen-binding protein, PPHalpha enolase like 1, tau-crystallin
Gene Symbol
ENO1
UniProt
Additional Enolase 1 Products
Product Documents for Enolase 1 Antibody
Product Specific Notices for Enolase 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.