Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15659
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9B4Y4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 47-100 of human Enolase 2/Neuron-specific Enolase (NP_001966.1). LELRDGDKQRYLGKGVLKAVDHINSTIAPALISSGLSVVEQEKLDNLMLELDGTENKSKFGANA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)
Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] -
Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Negative control (NC): U-937
Exposure time: 15s.
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] -
Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Confocal imaging of Neuro-2a cells using Enolase 2/Neuron-specific Enolase Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] -
Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.Applications for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:2000 - 1:10000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Enolase 2/Neuron-specific Enolase
Alternate Names
ENO2, gamma-Enolase, Neuronspecific Enolase, NSE
Gene Symbol
ENO2
Additional Enolase 2/Neuron-specific Enolase Products
Product Documents for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)
Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (9B4Y4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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