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Enolase 2/Neuron-specific Enolase Antibody (NSE-P1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30894

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-30894-200ug
NBP3-30894-50ug

Key Product Details

Species Reactivity

Human, Mouse, Chicken

Applications

ELISA, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # NSE-P1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human Enolase 2/Neuron-specific Enolase protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (NSE-P1)

Enolase 2/Neuron-specific Enolase Antibody (NSE-P1)

Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (NSE-P1) [NBP3-30894] -

Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (NSE-P1) [NBP3-30894] - Left and Center: Immunohistochemical staining of normal pancreas tissue (left) and small bowel tumor tissue (center) using NSE antibody (including NBP3-30894). Right: Western blot analysis using NSE antibodies (including NBP3-30894) on alpha and gamma enolase standards.

Applications for Enolase 2/Neuron-specific Enolase Antibody (NSE-P1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

: 1-5 ug/ml

Western Blot

: 1-2 ug/ml
Application Notes
.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Phosphate buffered saline

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (NSE-P1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (NSE-P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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