Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NSEP2

Format

BSA Free

Concentration

1 mg/ml

View all Enolase 2/Neuron-specific Enolase Antibodies »

Product Specifications

Immunogen

Ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P2: aa's 271-285 - TGDQLGALYQDFVRD

Specificity

Not reactive with -isozyme of enolase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (NSEP2)BSA Free [NBP2-50533]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (NSEP2)BSA Free [NBP2-50533]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (NSEP2) [NBP2-50533] - Lysates were separated on SDS-PAGE and probed with Anti-NSE [NSE-P2]. Lane 1: SH-SY5Y, Lane 2: NT2/D1.

Applications for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): Formalin-fixed, paraffin-embedded nerve tissue sections

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Gene Symbol

ENO2

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody (NSEP2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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