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Enolase 2/Neuron-specific Enolase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1606

Novus Biologicals, part of Bio-Techne
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NB100-1606

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Chickens were immunized with two synthetic peptide/keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Enolase 2/Neuron-specific Enolase gene product, but are shared between the human (NP_001966, NCBI) and rat (AAA41119, NCBI) sequences.

Reactivity Notes

NB 100-1606 reacts with mouse NSE type 2. The peptide sequences are shared between the human and rat sequences.

Marker

Neuron Cell Marker

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Enolase 2/Neuron-specific Enolase Antibody

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606]

Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Western blot showing a single band at the correct MW (47 kDa).

Applications for Enolase 2/Neuron-specific Enolase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10000-1:20000

Immunohistochemistry

1:10000-1:20000

Western Blot

1:10000-1:20000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (0.9% isotonic, w/v, pH 7.2)

Preservative

0.02% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Enolase 2/Neuron-specific Enolase

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Alternate Names

ENO2, gamma-Enolase, Neuronspecific Enolase, NSE

Entrez Gene IDs

2026 (Human); 13807 (Mouse); 24334 (Rat)

Gene Symbol

ENO2

UniProt

Additional Enolase 2/Neuron-specific Enolase Products

Product Documents for Enolase 2/Neuron-specific Enolase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Enolase 2/Neuron-specific Enolase Antibody

Chicken products cannot be exported to Canada.

Purification Notes
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.

Storage Notes
Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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