ENPP-2/Autotaxin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32162
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (92%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human ENPP-2/Autotaxin. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).
Localization
Secreted
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ENPP-2/Autotaxin Antibody
![Immunocytochemistry/ Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162]](https://resources.bio-techne.com/images/products/ENPP-2-Autotaxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32162-img0012.jpg "Immunocytochemistry/ Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162]")
Immunocytochemistry/ Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162]
Immunocytochemistry/Immunofluorescence: ENPP-2/Autotaxin Antibody [NBP1-32162] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Autotaxin protein stained by Autotaxin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: ENPP-2/Autotaxin Antibody [NBP1-32162] -
Western Blot: ENPP-2/Autotaxin Antibody [NBP1-32162] - Human Cerebrospinal Fluid (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ENPP-2/Autotaxin antibody (NBP1-32162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for ENPP-2/Autotaxin Antibody
Application
Recommended Usage
Flow Cytometry
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
Reported in scientific literature (PMID: 28739650)
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ENPP-2/Autotaxin
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 2
Alternate Names
ATX, Autotaxin, ENPP2, Lysophosphatidic Acid, NPP2, PDNP2
Gene Symbol
ENPP2
UniProt
Additional ENPP-2/Autotaxin Products
Product Documents for ENPP-2/Autotaxin Antibody
Product Specific Notices for ENPP-2/Autotaxin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov