EPB41L5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93392
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ENLPQSPGTDQHD, from the internal region of the protein sequence according to NP_065960.2; NP_001171866.1; NP_001171868.1; NP_001317239.1.
Epitope
C-ENLPQSPGTDQHD
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for EPB41L5 Antibody
Immunocytochemistry/Immunofluorescence: EPB41L5 Antibody [NB100-93392] -
Immunocytochemistry/Immunofluorescence: EPB41L5 Antibody [NB100-93392] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear, plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: EPB41L5 Antibody [NB100-93392] -
Flow Cytometry: EPB41L5 Antibody [NB100-93392] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: EPB41L5 Antibody [NB100-93392] -
Western Blot: EPB41L5 Antibody [NB100-93392] - (1ug/ml) staining of A431 (A) and (0.3ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.Applications for EPB41L5 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
0.3-1 ug/ml
Application Notes
WB: Approx. 80kDa band observed in lysates of cell line A431 and approx. 75kDa in lysates of cell line MCF7 (calculated MW of 81.9kDa according to NP_065960.2). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: EPB41L5
Alternate Names
band 4.1-like protein 5, erythrocyte membrane protein band 4.1 like 5, FLJ12957, KIAA1548BE37, YMO1
Entrez Gene IDs
57669 (Human)
Gene Symbol
EPB41L5
UniProt
Additional EPB41L5 Products
Product Documents for EPB41L5 Antibody
Product Specific Notices for EPB41L5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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