EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80722

Novus Biologicals, part of Bio-Techne
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NBP2-80722
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BSA Free
NBP2-22381
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 60N5D8

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all EpCAM/TROP1 Antibodies »

Product Summary for EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free

Immunogen

Within the range of amino acids 289-335 were used as the immunogen for the EpCAM antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free

Western Blot: EpCAM/TROP1 Antibody (60N5D8)Azide and BSA Free [NBP2-80722]

Western Blot: EpCAM/TROP1 Antibody (60N5D8)Azide and BSA Free [NBP2-80722]

Western Blot: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722] - Analysis of EpCAM in HT-29 lysate in the (A) absence and (B) presence of immunizing peptide using EpCAM antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Immunohistochemistry: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722]

Immunohistochemistry: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722]

Immunohistochemistry: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722] - FFPE human colon stained with EpCAM antibody (1:200), peroxidase-conjugate and DAB chromogen. Image from the standard format of this antibody.
Flow Cytometry: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722]

Flow Cytometry: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722]

Flow Cytometry: EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free [NBP2-80722] - Analysis of HT-29 cells using EpCAM antibody at 0.2 ug/10^6 cells. this antibody was used for this test. Top picture shows no cell surface staining seen, bottom shows intracellular staining of EpCAM (red represents EpCAM antibody and green represents isotype control). Image from the standard format of this antibody.

Applications for EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.2-1 ug/10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:400

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EpCAM/TROP1

Epithelial cell adhesion molecule (EpCAM), also known as TROP1 and CD326, is a type I transmembrane glycoprotein that plays a role in a number of cellular processes including adhesion, signaling, maintaining stemness, proliferation, migration, and invasion (1,2). The human EpCAM protein is 314 amino acids (aa) in length with a theoretical molecular weight (MW) of ~35 kDa (1,3,4). The EpCAM protein includes a signal peptide, an extracellular N-terminal domain, a thyroglobulin type-1 domain, a carboxyl-terminal domain, a single-pass transmembrane domain, and an intracellular domain (1,3). The protein is highly conserved and has approximately 81% aa sequence identity between human and mouse (5). Trophoblast cell-surface antigen 2 (TROP2) also shares ~67% aa sequence similarity with EpCAM (1,5). While both EpCAM and TROP2 are cell surface markers expressed in the epithelium, their expression levels typically do not correlate (5). EpCAM is expressed in normal epithelial tissue and elevated expression is observed in many epithelial carcinomas (1-3) Patient tumor samples with increased EpCAM expression have been associated with poor prognosis (1). Given EpCAM's elevated expression in tumors, it has become a potential target for cancer therapy approaches, including monoclonal antibody treatment and anti-EpCAM trispecific antibodies (1,5).

EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).

References

1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255

2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764

3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956

4. Uniprot (P16422)

5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Gene Symbol

EPCAM

Additional EpCAM/TROP1 Products

Product Documents for EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EpCAM/TROP1 Antibody (60N5D8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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