EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48290
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted [NBP2-48290]](https://resources.bio-techne.com/images/products/EpCAM-TROP1-Antibody-PAN-EpCAM-Cocktail-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48290-img0001.jpg "Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted [NBP2-48290]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PAN-EpCAM (Cocktail)
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted
Immunogen
Recombinant human EpCAM/TROP1 protein (full-length and fragments) (Uniprot: P16422 )
Localization
Cell surface
Specificity
It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or EpCAM. EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted [NBP2-48290]
Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted [NBP2-48290] - Human Breast Carcinoma stained with EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).Applications for EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: EpCAM/TROP1
EpCAM functions as an intracellular signaling molecule and contributes to regulation of epithelial-to-mesenchymal (EMT) transition (4,5). EpCAM is cleaved during the process of regulated intramembrane proteolysis (RIP) (4,5). Initial cleavage occurs at the membrane via a disintegrin and metalloprotease 17 (ADAM17) which releases EpCAM's extracellular domain (EpEx) (4,5). A secondary cleavage is mediated by presenilin 2 (PSEN2) which releases EpCAM's cytoplasmic trail (EpICD) (4,5). EpICD translocates to the nucleus where it associates with beta-catenin, FHL-2, and LEF-1 and induces transcription of genes related to EMT and tumor growth (4,5). EpCAM has also been shown to regulate structure and functionality of the apical junction complex of cells through direct interaction with claudin-7 and association with E-cadherin (2,5). Loss of EpCAM disrupts adherins junction and tight junction structure and function (2).
References
1. Mohtar MA, Syafruddin SE, Nasir SN, Low TY. Revisiting the Roles of Pro-Metastatic EpCAM in Cancer. Biomolecules. 2020; 10(2):255. https://doi.org/10.3390/biom10020255
2. Huang L, Yang Y, Yang F, et al. Functions of EpCAM in physiological processes and diseases (Review). Int J Mol Med. 2018; 42(4):1771-1785. https://doi.org/10.3892/ijmm.2018.3764
3. Brown TC, Sankpal NV, Gillanders WE. Functional Implications of the Dynamic Regulation of EpCAM during Epithelial-to-Mesenchymal Transition. Biomolecules. 2021; 11(7):956. https://doi.org/10.3390/biom11070956
4. Uniprot (P16422)
5. Schnell U, Cirulli V, Giepmans BN. EpCAM: structure and function in health and disease. Biochim Biophys Acta. 2013; 1828(8):1989-2001. https://doi.org/10.1016/j.bbamem.2013.04.018
Long Name
Epithelial Cell Adhesion Molecule
Alternate Names
17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1
Gene Symbol
EPCAM
Additional EpCAM/TROP1 Products
Product Documents for EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted
Product Specific Notices for EpCAM/TROP1 Antibody (PAN-EpCAM (Cocktail)) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...