EphA2 Antibody (1C1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52677
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Agonist Activity, CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 1C1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for EphA2 Antibody (1C1) - Chimeric - Azide and BSA Free
Immunogen
1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
Specificity
1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, EphA2, and EphA4, or any EphB receptor family members.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Recombinant monoclonal antibody to Human Ephrin Type A receptor 2. Manufactured using AbAb’s Recombinant Platform with variable regions (i.e. specificity) from the phage display antibody 1C1. 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Scientific Data Images for EphA2 Antibody (1C1) - Chimeric - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: EphA2 Antibody (1C1) [NBP2-52677] -
Immunocytochemistry/Immunofluorescence: EphA2 Antibody (1C1) [NBP2-52677] - Fixed murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 and a donkey anti-mouse IgG-AF488 for 90 mins. Nuclei stained with Hoechst. Panel A shows a merge of the fluorescence channels, whilst panel B shows the DIC image. In this fixed sample the cell membrane is clearly outlined.
Immunocytochemistry/Immunofluorescence: EphA2 Antibody (1C1) [NBP2-52677] -
Immunocytochemistry/Immunofluorescence: EphA2 Antibody (1C1) [NBP2-52677] - Live murine Lewis Lung cells were stained with the chimeric mouse IgG1 version of 1C1 and a donkey anti-mouse IgG-AF488 for 90 mins. Nuclei stained with Hoechst. Panel A shows the AF488 signal, panel B the DIC image and panel C a merge of fluorescence (AF488 + Hoechst) with the DIC image. In this live sample, antibody internalisation is observed.Applications for EphA2 Antibody (1C1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10 - 1:1000
Western Blot
1:100 - 1:2000
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human EphA2 receptor, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: EphA2
Alternate Names
Eck, Myk2, Sek2
Gene Symbol
EPHA2
Additional EphA2 Products
Product Documents for EphA2 Antibody (1C1) - Chimeric - Azide and BSA Free
Product Specific Notices for EphA2 Antibody (1C1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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