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EphB2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55398

Novus Biologicals, part of Bio-Techne
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NB100-55398

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-QKDRNHRPKFGQ corresponding to internal region according to NP_004433.2, NP_059145.2.

Epitope

QKDRNHRPKFGQ

Specificity

This antibody is expected to recognise both reported isoforms ( NP_059145.2 and NP_004433.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EphB2 Antibody

Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398] - Analysis of Human lung and placenta lysates using NB100-55398.
Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398]

Western Blot: EphB2 Antibody [NB100-55398] - (1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for EphB2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human lung and placenta lysates is reported in scientific literature (PMID: 17428795).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EphB2

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Long Name

Eph Receptor B2

Alternate Names

Cek5, Drt, Erk, Hek5, Nuk, Qek2, Sek3, Tyro5

Entrez Gene IDs

2048 (Human); 13844 (Mouse)

Gene Symbol

EPHB2

Additional EphB2 Products

Product Documents for EphB2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EphB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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