EphB3 Antibody (1B3)
Novus Biologicals, part of Bio-Techne | Catalog # H00002049-M01
![ELISA: EphB3 Antibody (1B3) [H00002049-M01]](https://resources.bio-techne.com/images/products/EphB3-Antibody-1B3-ELISA-H00002049-M01-img0014.jpg "ELISA: EphB3 Antibody (1B3) [H00002049-M01]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1B3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
EPHB3 (NP_004434, 899 a.a. ~ 997 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AASLKVIASAQSGMSQPLLDRTVPDYTTFTTVGDWLDAIKMGRYKESFVSAGFASFDLVAQMTAEDLLRIGVTLAGHQKKILSSIQDMRLQMNQTLPVQ
Specificity
EPHB3 - EPH receptor B3
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for EphB3 Antibody (1B3)
![Western Blot: EphB3 Antibody (1B3) [H00002049-M01]](https://resources.bio-techne.com/images/products/EphB3-Antibody-1B3-Western-Blot-H00002049-M01-img0013.jpg "Western Blot: EphB3 Antibody (1B3) [H00002049-M01]")
Western Blot: EphB3 Antibody (1B3) [H00002049-M01]
Western Blot: EphB3 Antibody (1B3) [H00002049-M01] - Analysis of EPHB3 expression in transfected 293T cell line by EPHB3 monoclonal antibody (M01), clone 1B3.Lane 1: EPHB3 transfected lysate(110.3 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: EphB3 Antibody (1B3) [H00002049-M01]](https://resources.bio-techne.com/images/products/EphB3-Antibody-1B3-Immunohistochemistry-Paraffin-H00002049-M01-img0010.jpg "Immunohistochemistry-Paraffin: EphB3 Antibody (1B3) [H00002049-M01]")
Immunohistochemistry-Paraffin: EphB3 Antibody (1B3) [H00002049-M01]
Immunohistochemistry-Paraffin: EphB3 Antibody (1B3) [H00002049-M01] - Analysis of monoclonal antibody to EPHB3 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.![Western Blot: EphB3 Antibody (1B3) [H00002049-M01]](https://resources.bio-techne.com/images/products/EphB3-Antibody-1B3-Western-Blot-H00002049-M01-img0012.jpg "Western Blot: EphB3 Antibody (1B3) [H00002049-M01]")
Western Blot: EphB3 Antibody (1B3) [H00002049-M01]
Western Blot: EphB3 Antibody (1B3) [H00002049-M01] - EPHB3 monoclonal antibody (M01), clone 1B3 Analysis of EPHB3 expression in HeLa.Applications for EphB3 Antibody (1B3)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: EphB3
Long Name
Eph Receptor B3
Alternate Names
Cek10, Hek2, Mdk5, Sek4, Tyro6
Entrez Gene IDs
2049 (Human)
Gene Symbol
EPHB3
OMIM
601839 (Human)
UniProt
Additional EphB3 Products
Product Documents for EphB3 Antibody (1B3)
Product Specific Notices for EphB3 Antibody (1B3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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