EphB3 Antibody (8H9P4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15693

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15693-20ul
NBP3-15693-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8H9P4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EphB3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human EphB3 (EPHB3) (P54753). SELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRRDVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWM

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EphB3 Antibody (8H9P4)

Western Blot: EphB3 Antibody (8H9P4) [NBP3-15693]

Western Blot: EphB3 Antibody (8H9P4) [NBP3-15693]

Western Blot: EphB3 Antibody (8H9P4) [NBP3-15693] - Western blot analysis of extracts of various cell lines, using EphB3 (EPHB3) antibody (NBP3-15693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Western Blot: EphB3 Antibody (8H9P4) [NBP3-15693]

Western Blot: EphB3 Antibody (8H9P4) [NBP3-15693]

Western Blot: EphB3 Antibody (8H9P4) [NBP3-15693] - Western blot analysis of extracts of Mouse brain, using EphB3 (EPHB3) antibody (NBP3-15693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for EphB3 Antibody (8H9P4)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EphB3

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Long Name

Eph Receptor B3

Alternate Names

Cek10, Hek2, Mdk5, Sek4, Tyro6

Gene Symbol

EPHB3

Additional EphB3 Products

Product Documents for EphB3 Antibody (8H9P4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EphB3 Antibody (8H9P4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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