EphB4 Antibody (S04-3B0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19861

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19861-100ul
NBP3-19861-50ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S04-3B0

Concentration

0.3 mg/ml

View all EphB4 Antibodies »

Product Specifications

Immunogen

Recombinant protein of human EphB4 (Uniprot # P54760)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

135 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EphB4 Antibody (S04-3B0)

Western Blot: EphB4 Antibody (S04-3B0) [NBP3-19861]

Western Blot: EphB4 Antibody (S04-3B0) [NBP3-19861]

Western Blot: EphB4 Antibody (S04-3B0) [NBP3-19861] - Western blot detection of EphB4 in Hela, A549, C6 cell lysates using NBP3-19861 (1:1000 diluted). Predicted band size: 108kDa. Observed band size: 135kDa.
Immunohistochemistry-Paraffin: EphB4 Antibody (S04-3B0) [NBP3-19861]

Immunohistochemistry-Paraffin: EphB4 Antibody (S04-3B0) [NBP3-19861]

Immunohistochemistry-Paraffin: EphB4 Antibody (S04-3B0) [NBP3-19861] - Immunohistochemistry of EphB4 in paraffin-embedded Human breast cancer tissue using NBP3-19861 at dilution 1/50

Applications for EphB4 Antibody (S04-3B0)

Application
Recommended Usage

Immunohistochemistry

1/100

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2, is a member of the Eph receptor family, which binds of the ephrin ligand family. Two classes of receptors exist, designated A and B, that have an extracellular domain made up of a globular domain, a cysteine-rich domain, and two fibronectin type III domains, followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains juxtamembrane motif with two tyrosines, which are the major autophosphorylation sites, along with a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxyl terminus, which includes one conserved tyrosine. Ligand recognition and binding leads to activation of intrinsic kinase activity. Only membrane-bound or Fc-clustered ligands have been shown to activate the receptor in vitro. Soluble monomeric ligands can bind the receptor, but do not induce receptor autophosphorylation and activation. The Eph receptors and ephrin ligands display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands.Ephs and ephrins play a significant role in angiogenesis.

Long Name

Eph Receptor B4

Alternate Names

Htk, Mdk2, Myk1, Tyro11

Gene Symbol

EPHB4

Additional EphB4 Products

Product Documents for EphB4 Antibody (S04-3B0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EphB4 Antibody (S04-3B0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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