Ephrin-A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52103

Novus Biologicals, part of Bio-Techne
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NBP1-52103
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Canine (100%), Human (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ephrin-A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KVTVSGKITHSP corresponding to internal region according to NP_004419.2.

Epitope

C-KVTVSGKITHSP

Specificity

This antibody is expected to recognize isoform a (NP_004419.2) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ephrin-A1 Antibody

Western Blot: Ephrin-A1 Antibody [NBP1-52103]

Western Blot: Ephrin-A1 Antibody [NBP1-52103]

Western Blot: Ephrin-A1 Antibody [NBP1-52103] - (0.01ug/ml) staining of Mouse Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Ephrin-A1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in mouse thymus and rat lung lysates (calculated MW of 23.8 kDa band according to mouse NP_034237.3 and rat NP_446051.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-A1

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNA class ephrin which binds to the EPHA2, EPHA4, EPHA5, EPHA6, and EPHA7 receptors. Two transcript variants that encode different isoforms were identified through sequence analysis.

Alternate Names

B61, EFL-1, EFNA1, EphrinA1, LERK1

Entrez Gene IDs

1942 (Human); 13636 (Mouse); 94268 (Rat)

Gene Symbol

EFNA1

UniProt

NP_004419.2 (Human)

Additional Ephrin-A1 Products

Product Documents for Ephrin-A1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ephrin-A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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