Ephrin-A2 Antibody (OTI3E3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02810

Novus Biologicals, part of Bio-Techne
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NBP2-02810
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E3

Concentration

1 mg/ml

View all Ephrin-A2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ephrin-A2 Antibody (OTI3E3)

Western Blot: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]

Western Blot: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]

Western Blot: Ephrin A2 Antibody (3E3) [NBP2-02810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ephrin A2.
Immunocytochemistry/Immunofluorescence: Ephrin A2 Antibody (3E3) [NBP2-02810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.
Immunohistochemistry-Paraffin: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]

Immunohistochemistry-Paraffin: Ephrin-A2 Antibody (OTI3E3) [NBP2-02810]

Immunohistochemistry-Paraffin: Ephrin A2 Antibody (3E3) [NBP2-02810] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.
View 1 more images

Applications for Ephrin-A2 Antibody (OTI3E3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-A2

This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq].

Alternate Names

Cek7-L, EFNA2, ELF-1, EphrinA2, LERK6

Entrez Gene IDs

1943 (Human)

Gene Symbol

EFNA2

Additional Ephrin-A2 Products

Product Documents for Ephrin-A2 Antibody (OTI3E3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ephrin-A2 Antibody (OTI3E3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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