Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70622

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI3E3

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Ephrin-A2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

Western Blot: Ephrin-A2 Antibody (OTI3E3)Azide and BSA Free [NBP2-70622]

Western Blot: Ephrin-A2 Antibody (OTI3E3)Azide and BSA Free [NBP2-70622]

Western Blot: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ephrin A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ephrin A2.
Immunocytochemistry/ Immunofluorescence: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622]

Immunocytochemistry/ Immunofluorescence: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622]

Immunocytochemistry/Immunofluorescence: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Ephrin A2.
Immunohistochemistry: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622]

Immunohistochemistry: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622]

Immunohistochemistry: Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free [NBP2-70622] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Ephrin A2 mouse monoclonal antibody.
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Applications for Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ephrin-A2

The Eph subfamily represents the largest group of receptor protein kinases identified to date. There is increasing evidence that Eph family members are involved in central nervous system function and in development. Ligands for Eph receptors include ephrin-A1 (LERK-1/B61), identified as a ligand for the EphA2 (Eck) receptor; ephrin-A2 (ELF-1), identified as a ligand for the EphA3 and EphA4 (Sek) receptors; ephrin-A3 (LERK-3), identified as a ligand for EphA5 (Ehk1) and EphA3 (Hek) receptors; ephrin-A4 (LERK-4), identified as a ligand for the EphA3 receptor; ephrin-A5 (AL-1), identified as a ligand for EphA5 (REK7); ephrin-B1 (LERK-2), identified as a ligand for the EphB1 (Elk) and EphB2 (Cek5) receptors; ephrin-B2 (LERK-5), identified as a ligand for the EphB1, EphB3 (Cek10) and EphB2 receptors; and ephrin-B3 (LERK-8), identified as a ligand for EphB1.

Alternate Names

Cek7-L, EFNA2, ELF-1, EphrinA2, LERK6

Gene Symbol

EFNA2

Additional Ephrin-A2 Products

Product Documents for Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ephrin-A2 Antibody (OTI3E3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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